CoA activated compounds

Quantification of coenzyme-A activated compounds

Detection and quantification of 11 analytes of the CoA metabolite class from tissue and cell culture.

Analytes

Acetyl-CoA

Malonyl-CoA

DL-Beta-Hydroxybutyryl

Lauroyl-CoA

Hexanoyl-CoA (C6:0)

Palmitoyl-CoA (C16:0)

Palmitoleoyl-CoA (C16:1)

Stearoyl-CoA (C18:0)

Oleoyl-CoA (C18:1)

Linoleoyl-CoA (C18:2)

Arachidonoyl-CoA (C20:4)

data type

absolute quantification by internal standardisation

Matrix

tissue samples, cell culture

Sample Extraction

Homogenisation

Protein precipitation

Analysis Techniques

LC/MS/MS

Analysis Instruments

TSQ Quantum, LTQ Orbitrap XL, Dionex UltiMate 3000

 

Reference:

Magnes et al. (2008): Validated comprehensive analytical method for quantification of coenzyme A activated compounds in biological tissues by online solid-phase extraction LC/MS/MS.