Energy metabolism

Energy metabolism

High-resolution mass spectrometry method, allowing the targeted analysis of more than 200 metabolites with the option for an untargeted analysis of all signals in the sample and the option to categorize and identify known unknowns.

Analytes   70-1100 m/z Full Scan and 70-600 m/z HCD-Scan
data typeDepending on the biological objectives internal isotope standard can be used for quantification
Matrixtissue, cell culture
Sample ExtractionProtocols adapted to cell type
Analysis TechniquesHESI (negative mode) LC/MS/MS (full ion fragmentation)
Analysis InstrumentsThermo Exactive, Dionex Ultimate 3000

 

Reference:

Trausinger et al. (2015): Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts