High-resolution mass spectrometry method, allowing the targeted analysis of more than 200 metabolites with the option for an untargeted analysis of all signals in the sample and the option to categorize and identify known unknowns.
|Analytes||70-1100 m/z Full Scan and 70-600 m/z HCD-Scan|
|data type||Depending on the biological objectives internal isotope standard can be used for quantification|
|Matrixt||issue, cell culture|
|Sample Extraction||Protocols adapted to cell type|
|Analysis Techniques||HESI (negative mode) LC/MS/MS (full ion fragmentation)|
|Analysis Instruments||Thermo Exactive, Dionex Ultimate 3000|