Metabolite finger- printing
Metabolite fingerprinting of blood and biological fluids
A high-resolution mass spectrometry method, allowing the targeted analysis of more than 200 metabolites with the option for an untargeted analysis of all signals in the sample.
Analytes | m/z 70 – 2000 in full scan or data dependent scan yields currently 170 known metabolites from plasma/serum |
data type | relative |
Matrix | Plasma, biological fluids |
Sample Volume | 25-500µl |
Sample Extraction | Precipitation with either Acetone, TCA or Methanol |
Analysis Techniques | LC/MS/MS |
Analysis Instruments | LTQ Orbitrap XL or Q-Exactive MS, Dionex Ultimate 3000 |
References:
Libiseller et al. (2015): IPO: a tool for automated optimization of XCMS parameters.