Spermidine

Quantification of spermidine and relatives

Targeting of Spermidine and related compounds (polyamines) allowing the quantification of derivated samples.

Analytes

N-Acetylputrescin

1,3-Diaminopropan

Putrescin

Cadaverin

L-Ornithin

Spermidine

d8-labelled Spermidin

N1-Acetylspermin

Spermine

data type

absolute quantification by internal standardisation

Matrix

blood, cell culture, tissue, biological fluids

Sample Extraction

Precipitation

Derivatisation

Analysis Techniques

LC/MS/MS

Analysis Instruments

TSQ Quantum, Dionex UltiMate 3000

 

Reference:

Magnes et al. (2014): Polyamines in biological samples: rapid and robust quantification by solid-phase extraction online-coupled to liquid chromatography-tandem mass spectrometry.